Journal: International Journal of Molecular Sciences

Article Title: The HIF-1α/EGF/EGFR Signaling Pathway Facilitates the Proliferation of Yak Alveolar Type II Epithelial Cells in Hypoxic Conditions

doi: 10.3390/ijms25031442

Figure Lengend Snippet: Effect of hypoxia on the proliferation of yak AT2 cells. ( A ) Diagram of the air-liquid culture model. ( B – E ) Protein expression of HIF-1α, EGF, and EGFR was measured after AT2 cells were exposed to normoxia or hypoxia for 12, 24, 36, 48, and 60 h. ( n = 3). ( F ) The STRING database was used to retrieve the targeting relationship between HIF-1a and EGFR signaling pathway factors. ( G , H ) Protein expression of PCNA was measured after AT2 cells were exposed to normoxia or hypoxia for 12, 24, 36, 48, and 60 h. ( n = 3). ( I , J ) After AT2 cells were exposed to normoxia or hypoxia for 12, 36, and 60 h, the expression of Ki67 and the number of positive cells were detected by immunofluorescence. ( n = 6). Microscope magnification is 20×. Data are means ± SD. ns stands for no difference, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Subsequently, the proteins were transferred to a polyvinylidene fluoride membrane and blocked with 5% skim milk at 24 °C for 2 h. The membrane was then incubated with polyclonal antibodies against SPC (1:1000; AP53886PU-N; OriGene, Maryland, USA), HIF-1α (1:1000; AF1009; Affinity, Changzhou, China), EGFR (1:300; bs-10007R; Bioss, Beijing, China), EGF (1:300; bs2010R; Bioss Beijing China), PCNA (1:300; bs-2006R; Bioss Beijing China), Bax (1:300; bs-20386R; Bioss Beijing China), and β-actin (1:2000, Bioss, Beijing, China).

Techniques: Expressing, Immunofluorescence, Microscopy

Journal: International Journal of Molecular Sciences

Article Title: The HIF-1α/EGF/EGFR Signaling Pathway Facilitates the Proliferation of Yak Alveolar Type II Epithelial Cells in Hypoxic Conditions

doi: 10.3390/ijms25031442

Figure Lengend Snippet: ML228 activates the HIF signaling pathway to enhance the proliferation of yak AT2 cells. ( A , B ) The cytotoxicity of DMOG and ML228 on AT2 cells was assessed using a CCK-8 assay. ( C ) Western blot analysis was performed to evaluate the impact of different concentrations of ML228 on the protein levels of HIF-1α, EGFR, EGF, and PCNA in AT2 cells. ( D – G ) Gray value analysis was conducted for Western blot results depicting expression levels of HIF-1α, EGFR, EGF, and PCNA proteins ( n = 3). ( H , I ) Immunofluorescence staining revealed the quantification of Ki67-positive (red) cells in AT2 cells following treatment with varying concentrations of ML228 ( n = 6). (bar = 100 μm). DAPI was used to stain the nucleus (blue). Data are means ± SD. ns stands for no difference, * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Subsequently, the proteins were transferred to a polyvinylidene fluoride membrane and blocked with 5% skim milk at 24 °C for 2 h. The membrane was then incubated with polyclonal antibodies against SPC (1:1000; AP53886PU-N; OriGene, Maryland, USA), HIF-1α (1:1000; AF1009; Affinity, Changzhou, China), EGFR (1:300; bs-10007R; Bioss, Beijing, China), EGF (1:300; bs2010R; Bioss Beijing China), PCNA (1:300; bs-2006R; Bioss Beijing China), Bax (1:300; bs-20386R; Bioss Beijing China), and β-actin (1:2000, Bioss, Beijing, China).

Techniques: CCK-8 Assay, Western Blot, Expressing, Immunofluorescence, Staining

Journal: Stem Cell Research & Therapy

Article Title: Mesenchymal stem cells promote pancreatic β-cell regeneration through downregulation of FoxO1 pathway

doi: 10.1186/s13287-020-02007-9

Figure Lengend Snippet: Primary and secondary antibodies list

Article Snippet: Primary antibody , Rabbit EGF antibody, polyclonal , 1:500 , Bioss.

Techniques:

Journal: Microbial Biotechnology

Article Title: Combination of an engineered Lactococcus lactis expressing CXCL12 with light‐emitting diode yellow light as a treatment for scalded skin in mice

doi: 10.1111/1751-7915.13885

Figure Lengend Snippet: Re‐epithelialization of wound skin after treatment with engineered strains combined with light. A. Skin wounds were IHC‐stained for Ki67 + and EGF populations of the wounds in the C, L, LC, Y, LY and LCY groups on the 9 day post‐scald ( n = 4 per group). The bar corresponds to 100 μm. B. C. The relative protein expressions of β‐catenin, c‐Myc, Wnt1, Jagged 1, Notch 1 and Hes 1 compared with GAPDH were analysed by Western blot of the wounds in the C, L, LC, Y, LY and LCY groups on the 9 day post‐scald and normal mice ( n = 4 per group; one‐way ANOVA Kruskal–Wallis test, * P < 0.05, ** P < 0.001, *** P < 0.0005).

Article Snippet: For immunohistochemistry (IHC) staining, primary antibodies anti‐Ki67 (1:200; Servicebio, Wuhan, China; Cat# GB111499) and anti‐EGF (1:200; Bioss, Beijing, China; Cat# bs‐2009R) were used.

Techniques: Staining, Western Blot